Teko ea Marang-rang e netefatsa hore litaba tsa substrate li ka tlase ho moeli, ho netefatsa ts'ebetso ea enzyme ea bioloji nakong eohle ea ts'ebetso, le ho eketsa sekhahla sa karabelo ea hydrolysis.
Lik'hemik'hale tsa Amide ke li-organic synthesis intermediates le lik'hemik'hale tsa bohlokoa 'me li sebelisoa haholo meriana, chefo e bolaeang likokoanyana, lijo, tšireletso ea tikoloho, tlhahiso ea oli le masimo a mang.Karabelo ea hydrolysis ea sehlopha sa nitrile ho sehlopha sa amide ke o mong oa mekhoa ea bohlokoa ea ho lokisa metsoako ea amide indastering.
Biocatalyst e sebelisoa ts'ebetsong ea motsoako o motala oa motsoako o itseng oa amide, 'me mosebetsi oa eona o ameha haholo ke khatello ea substrate le sehlahisoa tsamaisong.Haeba motsoako oa substrate o phahame haholo, mokelikeli o tla koaloa habonolo, o etsa hore ho se khonehe ho tsoela pele ka karabelo ea motsoako;haeba mohopolo oa sehlahisoa o phahame haholo, o tla lebisa ho bokelleng ha substrate le katleho e tlase ea synthesis.E le ho netefatsa ts'ebetso e nepahetseng ea li-catalysts tsa enzyme ea bioloji karabelong ea motsoako, ho hlokahala mekhoa e sebetsang ea tekheniki ho beha leihlo le ho fana ka maikutlo ho lokisa likhakanyo tsa nitrile substrates le lihlahisoa tsa amide ka nako ea nnete nakong ea karabelo.
Hajoale, mekhoa e joalo ka lisampole ka linako tse tsitsitseng le ho etsa likhakanyo tsa khase ea chromatography-mass spectrometry kamora sampole ea pele ho kalafo hangata e sebelisoa ho bona karoloana le lihlahisoa tsa sehlahisoa tsamaisong ea karabelo.Liphetho tsa ho sibolloa ha Marang-rang li lieha, boemo ba hona joale bo ke ke ba tsejoa ka nako ea nnete, 'me ho thata ho laola maikutlo le ho lokisa litaba tsa substrate,' me monyetla o motle oa ho fepa o ka fetoa.Theknoloji ea tlhahlobo ea spectral ea inthaneteng e na le melemo ea lebelo la ho lemoha kapele mme ha ho hlokahale hore ho be le mehlala ea tlhahlobo ea pele.E ka hlokomela tlhahlobo e potlakileng, ea nako ea nnete, ea in-situ le e bohlale ea sistimi ea karabelo, mme e na le melemo e ikhethang ho botala bo botala ba metsoako ea amide.
Setšoantšo se ka holimo se bontša tlhahlobo ea inthaneteng ea mokhoa oa ho lokisa acrylamide ka karabelo ea bioenzymatic ea motsoako o itseng oa nitrile.Ho tloha ho 0 ho isa ho t1 kamora hore karabelo e qale, sekhahla sa phepelo sa lisebelisoa tse tala tsa nitrile se batla se le seholo, 'me sekhahla sa ho bokellana ha substrate le sehlahisoa se batla se potlakile.Ho t1, substrate content e haufi le moeli o ka holimo oa monyako.Ka nako ena, basebetsi ba fokotsa sekhahla sa phepelo ea lihlahisoa tse tala ho boloka mahloriso a substrate ka har'a sistimi e laoloang, mme sehlahisoa se ntse se ka bokellana kapele.Qetellong, ha karabelo e tsoela pele ho nako ea t2, lihlahisoa tsa lihlahisoa li bokellana ho ea boemong bo reretsoeng, 'me basebeletsi ba tlhahiso ba khaotsa ho eketsa lisebelisoa tse tala tsa nitrile.Ka mor'a moo, boemo ba substrate bo atamela zero 'me lihlahisoa tsa lihlahisoa le tsona li atisa ho tsitsa.Nakong ea ts'ebetso eohle e tsoelang pele ea tlhahiso, ho beha leihlo marang-rang ho netefatsa hore karabelo ea enzyme ea tlhaho e tsoela pele hantle.
Ka kakaretso e kholo, theknoloji ea ho beha leihlo inthaneteng e bohlokoa haholo.Tsebo ea nako ea 'nete ea likarolo tsa substrate le lihlahisoa tsa lihlahisoa li ka thusa maikutlo ho fetola litaba tsa substrate ka har'a mefuta e loketseng.Nakong ea ts'ebetso ea karabelo, e ka eketsa ts'ebetso ea enzyme ea tlhaho, ea ntlafatsa ts'ebetso ea karabelo ea motsoako, 'me ea thusa ho laola liparamente tsa ts'ebetso maemong a nepahetseng.Eketsa bophelo ba ts'ebeletso ea li-catalyst tsa enzyme ea bioloji le ho holisa melemo.
Nako ea poso: Jan-23-2024